Facilities
The Exercise Physiology and Biochemistry Laboratory (EPBL) is located in the 3000 sq. ft. Exercise Physiology Laboratory. Inside our biochemistry and physiology laboratory you will find that we have a fully functional lab with dedicated facilities for cell culture, mouse holding (70 individually ventilated cage system and 30 activity cages), biochemical analysis, and sample storage. These facilities support the EBL’s focus on the study of physical inactivity, appetite regulation, obesity, diabetes, and atherosclerosis.
Equipment:
Two Ultra-low -80°C freezer for sample storage
4°C Laboratory refrigerator
Large capacity -20°C freezer
Millipore Direct-Q ultrapure water system
BD Biosciences Accuri C6 Flow Cytometer
ThermoFisher cryogenic (cell storage) vessel
ThermoFisher Sorvall ST40 refrigerated centrifuge
ThermoFisher Sorvall Legend Micro21 refrigerated centrifuge
Dry block incubator
Assorted vortex mixers
Incubating/shaking microplate shaker
Heated waterbath
Orbital blot shaker
Inverting mixer
Various homogenizers
Luminex MAGPIX System with xPONENT 4.2 analyzer
BioTek Epoch 2 microplate reader
BioTek Magnetic/Vacuum Washer ELx405TS
Bio-Rad 1575 microplate washer
STAT YSI-3000 blood lactate/glucose analyzer
Nitrogen heated evaporator
Applied Biosystems 7500 fast RT-PCR and Verti-Thermocycler
Microplate centrifuge
Invitrogen Qubit 2.0 flurometer
Chemical and flammable liquid storage cabinets
Bio-Rad Power Supply and Gel Blot Electrophoresis System
Bio-Rad ChemiDoc MP system
Bio-Rad Trans-Blot Turbo transfer system
Millipore SNAP id 2.0
ThermoScientific CO2 incubator
ThermoScientific 1300 class II type A2 biological safety cabinet
Ductless fume hood
Mettler Toledo analytical balance
Mettler Toledo pH meter
Leica CM1850 Cryostat
Leica disecting light microscope with digital camera
Carl Zeiss inverted light microscope
Amscope dissecting light microscope with digital camera
Nikon fluorescent microscope with digital camera
Joshua Wooten, Ph.D.
Professor of Exercise Physiology