Reverse the direction and set the flow rate to be 0.5mL/min (KW-800 series and LW-803) or 0.15mL/min (KW400) series).

The volume of the cleaning solvent required is 5 to 10 times the column volume. ,p> 1) In case where protein has been adsorbed:

Use a solvent containing 0.5M salt or phosphate buffer (about pH3.0).

2) In case where a hydrophobic substance has been adsorbed :

Use a solvent containing 10 to 20% acetonitrile or methanol.